Charlotte Uetrecht presents: FLYING VIRUSES – FROM BIOPHYSICAL TO STRUCTURAL CHARACTERIZATION
July 15, 2021 11:00 AM Eastern Time (US and Canada)
An integral part of icosahedral viruses is the capsid protein shell protecting the genome, where many protein copies self-assemble into shells of defined size. These capsids can be studied by native and hydrogen/deuterium exchange mass spectrometry (MS) in terms of stoichiometry, dynamics, assembly pathways and stability to investigate strain specific traits of noroviruses, the main cause of viral gastroenteritis. Moreover, the highly dynamic replication machinery of coronaviruses has been a longstanding interest and newest results on various viruses including SARS-CoV-2 will be presented. Despite the remarkable sensitivity, the structural resolution is limited in native MS, and a short preview on native MS for single particle imaging at X-ray free-electron-lasers will be provided.