Here are a number of relevant publications on top-down methods and applications.  Use the search box or browse all below.

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Wei B, Zenaidee MA, Lantz C, Ogorzalek Loo RR, Loo JA. 2021. Towards understanding the formation of internal fragments generated by collisionally activated dissociation for top-down mass spectrometry. Analytica Chimica Acta. 339400
Lefebvre D, Fenaille F, Merda D, Blanco-Valle K, Feraudet-Tarisse C, et al. 2021. Top-Down Mass Spectrometry for Trace Level Quantification of Staphylococcal Enterotoxin A Variants. J. Proteome Res.
Nagy C, Andrási M, Hamidli N, Gyémánt G, Gáspár A. 2021. Top-down proteomic analysis of monoclonal antibodies by capillary zone electrophoresis - mass spectrometry. Journal of Chromatography Open. 100024
Kline JT, Mullen C, Durbin KR, Oates RN, Huguet R, et al. 2021. Sequential Ion–Ion Reactions for Enhanced Gas-Phase Sequencing of Large Intact Proteins in a Tribrid Orbitrap Mass Spectrometer. J. Am. Soc. Mass Spectrom. 32(9):2334–45
Jeanne Dit Fouque K, Kaplan D, Voinov VG, Holck FHV, Jensen ON, Fernandez-Lima F. 2021. Proteoform Differentiation using Tandem Trapped Ion Mobility, Electron Capture Dissociation, and ToF Mass Spectrometry. Anal. Chem.
Weisbrod CR, Anderson LC, Hendrickson CL, Schaffer LV, Shortreed MR, et al. 2021. Advanced Strategies for Proton-Transfer Reactions Coupled with Parallel Ion Parking on a 21 T FT-ICR MS for Intact Protein Analysis. Anal. Chem.
Rommelfanger SR, Zhou M, Shaghasi H, Tzeng S-C, Evans BS, et al. 2021. An Improved Top-Down Mass Spectrometry Characterization of Chlamydomonas reinhardtii Histones and Their Post-translational Modifications. J. Am. Soc. Mass Spectrom.
Melby JA, Roberts DS, Larson EJ, Brown KA, Bayne EF, et al. 2021. Novel Strategies to Address the Challenges in Top-Down Proteomics. J. Am. Soc. Mass Spectrom. 32(6):1278–94
Barbu IM, Lamers R-JAN, Gerritsen HW, Blokland MH, Bremer MGEG, Alewijn M. 2021. Endogenous protein and peptide analysis with LC-MS/(MS): A feasibility study for authentication of raw-milk farmer's cheese. International Dairy Journal. 117:104990
Luise A, De Cecco E, Ponzini E, Sollazzo M, Mauri P, et al. 2021. Profiling Dopamine-Induced Oxidized Proteoforms of β-synuclein by Top-Down Mass Spectrometry. Antioxidants. 10(6):893
Arauz-Garofalo G, Jodar M, Vilanova M, de la Iglesia Rodriguez A, Castillo J, et al. 2021. Protamine Characterization by Top-Down Proteomics: Boosting Proteoform Identification with DBSCAN. Proteomes. 9(2):21
Beckman JS, Voinov VG, Hare M, Sturgeon D, Vasil'ev Y, et al. 2021. Improved Protein and PTM Characterization with a Practical Electron-Based Fragmentation on Q-TOF Instruments. J. Am. Soc. Mass Spectrom.
Römer J, Stolz A, Kiessig S, Moritz B, Neusüß C. 2021. Online Top-down Mass Spectrometric Identification of CE(SDS)-separated Antibody Fragments by Two-dimensional Capillary Electrophoresis. Journal of Pharmaceutical and Biomedical Analysis. 114089
Borges Lima D, Dupré M, Mariano Santos MD, Carvalho PC, Chamot-Rooke J. 2021. DiagnoTop: A Computational Pipeline for Discriminating Bacterial Pathogens without Database Search. J. Am. Soc. Mass Spectrom.
Gerbasi VR, Melani RD, Abbatiello SE, Belford MW, Huguet R, et al. 2021. Deeper Protein Identification Using Field Asymmetric Ion Mobility Spectrometry in Top-Down Proteomics. Anal. Chem.
Lantz C, Zenaidee MA, Wei B, Hemminger Z, Ogorzalek Loo RR, Loo JA. 2021. ClipsMS: An Algorithm for Analyzing Internal Fragments Resulting from Top-Down Mass Spectrometry. J. Proteome Res. 20(4):1928–35
Simanjuntak Y, Schamoni-Kast K, Grün A, Uetrecht C, Scaturro P. 2021. Top-Down and Bottom-Up Proteomics Methods to Study RNA Virus Biology. Viruses. 13(4):668
Choi IK, Jiang T, Kankara SR, Wu S, Liu X. 2021. TopMSV: A Web-Based Tool for Top-Down Mass Spectrometry Data Visualization. J. Am. Soc. Mass Spectrom.
Schachner LF, Tran DP, Lee A, McGee JP, Jooss K, et al. 2021. Reassembling protein complexes after controlled disassembly by top-down mass spectrometry in native mode. International Journal of Mass Spectrometry. 116591
Lu L, Scalf M, Shortreed MR, Smith LM. 2021. Mesh Fragmentation Improves Dissociation Efficiency in Top-down Proteomics. J. Am. Soc. Mass Spectrom.
Yu D, Wang Z, Cupp-Sutton KA, Guo Y, Kou Q, et al. 2021. Quantitative Top-Down Proteomics in Complex Samples Using Protein-Level Tandem Mass Tag Labeling. J. Am. Soc. Mass Spectrom.
Brunner AM, Lössl P, Geurink PP, Ovaa H, Albanese P, et al. 2021. Quantifying Positional Isomers (QPI) by top-down mass spectrometry. Molecular & Cellular Proteomics. 100070
Roberts DS, Mann MW, Melby JA, Larson EJ, Zhu Y, et al. 2021. Structural O-Glycoform Heterogeneity of the SARS-CoV-2 Spike Protein Receptor-Binding Domain Revealed by Native Top-Down Mass Spectrometry. bioRxiv. 2021.02.28.433291
Zhang Z, Hug C, Tao Y, Bitsch F, Yang Y. 2021. Solving Complex Biologics Truncation Problems by Top-Down Mass Spectrometry. J. Am. Soc. Mass Spectrom.
Schaffer LV, Anderson LC, Butcher DS, Shortreed MR, Miller RM, et al. 2021. Construction of Human Proteoform Families from 21 Tesla Fourier Transform Ion Cyclotron Resonance Mass Spectrometry Top-Down Proteomic Data. J. Proteome Res. 20(1):317–25
Smith LM, Agar JN, Chamot-Rooke J, Danis PO, Ge Y, et al. 2021. The Human Proteoform Project: Defining the human proteome. Science Advances. 7(46):
Lermyte F. 2021. Top-Down Mass Spectrometry of Intact Proteins and Complexes: From Rare to Routine. Chromatography Online.
Nickerson JL, Baghalabadi V, Rajendran SRCK, Jakubec PJ, Said H, et al. 2021. Recent advances in top-down proteome sample processing ahead of MS analysis. Mass Spectrometry Reviews. n/a(n/a):


Brown KA, Melby JA, Roberts DS, Ge Y. 2020. Top-down Proteomics: Challenges, Innovations, and Applications in Basic and Clinical Research. Expert Review of Proteomics. 0(ja):null
Gadkari VV, Ramírez CR, Vallejo DD, Kurulugama RT, Fjeldsted JC, Ruotolo BT. 2020. Enhanced Collision Induced Unfolding and Electron Capture Dissociation of Native-like Protein Ions. Anal. Chem.
Zhou M, Lantz C, Brown KA, Ge Y, Paša-Tolić L, et al. 2020. Higher-order structural characterisation of native proteins and complexes by top-down mass spectrometry. Chem. Sci.
Dupré M, Duchateau M, Malosse C, Lima DB, Calvaresi V, et al. 2020. Optimization of a Top-Down Proteomics Platform for Closely-Related Pathogenic Bacteria Discrimination. J. Proteome Res.
Takemori A, Butcher DS, Harman VM, Brownridge P, Shima K, et al. 2020. PEPPI-MS: Polyacrylamide-Gel-Based Prefractionation for Analysis of Intact Proteoforms and Protein Complexes by Mass Spectrometry. J. Proteome Res. 19(9):3779–91
Zenaidee MA, Lantz C, Perkins T, Jung W, Loo RRO, Loo JA. 2020. Internal Fragments Generated by Electron Ionization Dissociation Enhance Protein Top-Down Mass Spectrometry. J. Am. Soc. Mass Spectrom. 31(9):1896–1902
Srzentić K, Fornelli L, Tsybin YO, Loo JA, Seckler H, et al. 2020. Interlaboratory Study for Characterizing Monoclonal Antibodies by Top-Down and Middle-Down Mass Spectrometry. J. Am. Soc. Mass Spectrom. 31(9):1783–1802
Weisbrod CR, Anderson LC, Greer JB, DeHart CJ, Hendrickson CL. 2020. Increased Single Spectrum Top-Down Protein Sequence Coverage in Trapping Mass Spectrometers with Chimeric Ion Loading. Anal. Chem.
Tiambeng TN, Roberts DS, Brown KA, Zhu Y, Chen B, et al. 2020. Nanoproteomics enables proteoform-resolved analysis of low-abundance proteins in human serum. Nat Commun. 11(1):1–12
Wu Z, Roberts DS, Melby JA, Wenger K, Wetzel M, et al. 2020. MASH Explorer: A Universal Software Environment for Top-Down Proteomics. J. Proteome Res.
Zenaidee MA, Lantz C, Perkins T, Jung W, Loo RRO, Loo JA. 2020. Internal Fragments Generated by Electron Ionization Dissociation Enhance Protein Top-Down Mass Spectrometry. J. Am. Soc. Mass Spectrom.
Choi IK, Abeysinghe E, Coulter E, Marru S, Pierce M, Liu X. 2020. TopPIC Gateway: A Web Gateway for Top-Down Mass Spectrometry Data Interpretation. Practice and Experience in Advanced Research Computing, pp. 461–64. Portland, OR, USA: Association for Computing Machinery
Lodge JM, Schauer KL, Brademan DR, Riley NM, Shishkova E, et al. 2020. Top-Down Characterization of an Intact Monoclonal Antibody using Activated Ion-Electron Transfer Dissociation. Anal. Chem.
Schaffer LV, Millikin RJ, Shortreed MR, Scalf M, Smith LM. 2020. Improving Proteoform Identifications in Complex Systems Through Integration of Bottom-Up and Top-Down Data. J. Proteome Res.
Serrao S, Firinu D, Olianas A, Deidda M, Contini C, et al. 2020. Top-down proteomics of human saliva discloses significant variations of the protein profile in patients with mastocytosis. J. Proteome Res.
Ives A, Su T, Durbin KR, Early BP, dos Santos Seckler H, et al. 2020. Using 10,000 fragment ions to inform scoring in native top-down proteomics. J. Am. Soc. Mass Spectrom.
Zhou M, Uwugiaren N, Williams SM, Moore RJ, Zhao R, et al. 2020. Sensitive Top-Down Proteomics Analysis of a Low Number of Mammalian Cells Using a Nanodroplet Sample Processing Platform. Anal. Chem.
DeHart CJ, Anderson LC, Adams LM, Fornelli L, Hendrickson CL, Kelleher NL. 2020. Abstract A26: Precise characterization and comparison of KRAS proteoforms by top-down mass spectrometry. Mol Cancer Res. 18(5 Supplement):A26–A26
Gault J, Liko I, Landreh M, Shutin D, Bolla JR, et al. 2020. Combining native and 'omics' mass spectrometry to identify endogenous ligands bound to membrane proteins. Nat Methods. 17(5):505–8
McIlwain SJ, Wu Z, Wetzel M, Belongia D, Jin Y, et al. 2020. Enhancing Top-Down Proteomics Data Analysis by Combining Deconvolution Results through a Machine Learning Strategy. J. Am. Soc. Mass Spectrom.
Polasky DA, Dixit SM, Keating MF, Gadkari VV, Andrews PC, Ruotolo BT. 2020. Pervasive Charge Solvation Permeates Native-like Protein Ions and Dramatically Influences Top-down Sequencing Data. J. Am. Chem. Soc.
Jeong K, Kim J, Gaikwad M, Hidayah SN, Heikaus L, et al. 2020. FLASHDeconv: Ultrafast, High-Quality Feature Deconvolution for Top-Down Proteomics. cels. 10(2):213-218.e6
McGee JP, Melani RD, Goodwin M, McAlister G, Huguet R, et al. 2020. Voltage Rollercoaster Filtering of Low-Mass Contaminants During Native Protein Analysis. J. Am. Soc. Mass Spectrom.
Coradin M, Mendoza MR, Sidoli S, Alpert AJ, Lu C, Garcia BA. 2020. Bullet points to evaluate the performance of the middle-down proteomics workflow for histone modification analysis. Methods
Chen D, Lubeckyj RA, Yang Z, McCool EN, Shen X, et al. 2020. Predicting Electrophoretic Mobility of Proteoforms for Large-Scale Top-Down Proteomics. Anal. Chem.
Kafader JO, Durbin KR, Melani RD, Des Soye BJ, Schachner LF, et al. 2020. Individual Ion Mass Spectrometry Enhances the Sensitivity and Sequence Coverage of Top-Down Mass Spectrometry. J. Proteome Res.
He L, Rockwood AL, Agarwal AM, Anderson LC, Weisbrod CR, et al. 2020. Top-down proteomics—a near-future technique for clinical diagnosis? Ann Transl Med. 8(4):
Gallagher K, Palasser Michael, Hughes S, Mackay CL, Kilgour DPA, Clarke DJ. 2020. Isotope Depletion Mass Spectrometry (ID-MS) for accurate mass determination and improved top-down sequence coverage of intact proteins. J. Am. Soc. Mass Spectrom.
Giorgetti J, Beck A, Leize-Wagner E, François Y-N. 2020. Combination of intact, middle-up and bottom-up levels to characterize 7 therapeutic monoclonal antibodies by capillary electrophoresis – mass spectrometry. Journal of Pharmaceutical and Biomedical Analysis. 113107
DelGuidice CE, Ismaiel OA, Mylott Jr WR, Halquist MS. 2020. Quantitative Bioanalysis of Intact Large Molecules using Mass Spectrometry. J Appl Bioanal. 6(1):52–64
Vimer S, Ben-Nissan G, Sharon M. 2020. Direct characterization of overproduced proteins by native mass spectrometry. Nat Protoc. 1–30
Shliaha PV, Gorshkov V, Kovalchuk SI, Schwämmle V, Baird MA, et al. 2020. Middle-Down Proteomic Analyses with Ion Mobility Separations of Endogenous Isomeric Proteoforms. Anal. Chem.
Dubois C, Payen D, Simon S, Junot C, Fenaille F, et al. 2020. Top-down and bottom-up proteomics of circulating S100A8/S100A9 in plasma of septic shock patients. J. Proteome Res.


Wu Z, Jin Y, Chen B, Gugger MK, Wilkinson-Johnson CL, et al. 2019. Comprehensive Characterization of the Recombinant Catalytic Subunit of cAMP-Dependent Protein Kinase by Top-Down Mass Spectrometry. J. Am. Soc. Mass Spectrom.
Vincent D, Binos S, Rochfort S, Spangenberg G. 2019. Top-Down Proteomics of Medicinal Cannabis. Proteomes. 7(4):33
Wilkins John T, Seckler Henrique, Rink Jonathan S, Thaxton Colby, Compton Philip, et al. 2019. Associations Between Apolipoprotein A-I Proteoforms and Markers of Cardiometabolic Health. Circulation. 140(Suppl_1):A15086–A15086
Holt MV, Wang T, Young NL. 2019. High-Throughput Quantitative Top-Down Proteomics: Histone H4. J. Am. Soc. Mass Spectrom.
Huguet R, Mullen C, Srzentić K, Greer JB, Fellers RT, et al. 2019. Proton transfer charge reduction enables high-throughput top-down analysis of large proteoforms. Anal. Chem.
Cai Wenxuan, Zhang Jianhua, de Lange Willem J., Gregorich Zachery R., Karp Hannah, et al. 2019. An Unbiased Proteomics Method to Assess the Maturation of Human Pluripotent Stem Cell–Derived Cardiomyocytes. Circulation Research. 125(11):936–53
Melby JA, Jin Y, Lin Z, Tucholski T, Wu Z, et al. 2019. Top-Down Proteomics Reveals Myofilament Proteoform Heterogeneity among Various Rat Skeletal Muscle Tissues. J. Proteome Res.
Melani RD, Srzentić K, Gerbasi VR, McGee JP, Huguet R, et al. 2019. Direct measurement of light and heavy antibody chains using ion mobility and middle-down mass spectrometry. mAbs. 0(0):1–7
Chen D, Geis-Asteggiante L, Gomes FP, Ostrand-Rosenberg S, Fenselau C. 2019. Top-down Proteomic Characterization of Truncated Proteoforms. J. Proteome Res.
Dai Y, Buxton KE, Schaffer LV, Miller RM, Millikin RJ, et al. 2019. Constructing Human Proteoform Families Using Intact-Mass and Top-Down Proteomics with a Multi-Protease Global PTM Discovery Database. J. Proteome Res.
O'Rourke MB, Town SEL, Dalla PV, Bicknell F, Koh Belic N, et al. 2019. What is Normalization? The Strategies Employed in Top-Down and Bottom-Up Proteome Analysis Workflows. Proteomes. 7(3):29
Shen X, Yang Z, McCool EN, Lubeckyj RA, Chen D, Sun L. 2019. Capillary zone electrophoresis-mass spectrometry for top-down proteomics. TrAC Trends in Analytical Chemistry. 115644
Smith LM, Thomas PM, Shortreed MR, Schaffer LV, Fellers RT, et al. 2019. A five-level classification system for proteoform identifications. Nat Methods. 1–2
Basharat AR, Iman K, Khalid MF, Anwar Z, Hussain R, et al. 2019. SPECTRUM – A MATLAB Toolbox for Proteoform Identification from Top-Down Proteomics Data. Sci Rep. 9(1):1–14
Donnelly DP, Rawlins CM, DeHart CJ, Fornelli L, Schachner LF, et al. 2019. Best practices and benchmarks for intact protein analysis for top-down mass spectrometry. Nature Methods. 16(7):587
Dowling P, Zweyer M, Swandulla D, Ohlendieck K. 2019. Characterization of Contractile Proteins from Skeletal Muscle Using Gel-Based Top-Down Proteomics. Proteomes. 7(2):25
Lermyte F, Tsybin YO, O'Connor PB, Loo JA. 2019. Top or Middle? Up or Down? Toward a Standard Lexicon for Protein Top-Down and Allied Mass Spectrometry Approaches. J. Am. Soc. Mass Spectrom.
Gregorich ZR, Patel JR, Cai W, Lin Z, Heurer R, et al. 2019. Deletion of Enigma Homologue from the Z-disc slows tension development kinetics in mouse myocardium. The Journal of General Physiology. 151(5):670–79
Pu Y, Chen Y, Nguyen T, Xu C-F, Zang L, et al. 2019. Application of a label-free and domain-specific free thiol method in monoclonal antibody characterization. Journal of Chromatography B. 1114–1115:93–99
Brown KA, Chen B, Guardado-Alvarez TM, Lin Z, Hwang L, et al. 2019. A photocleavable surfactant for top-down proteomics. Nature Methods. 1
LeDuc RD, Fellers RT, Early BP, Greer JB, Shams DP, et al. 2019. Accurate Estimation of Context-Dependent False Discovery Rates in Top-Down Proteomics. Mol. Cell Proteomics. 18(4):796–805
Jin Y, Diffee GM, Colman RJ, Anderson RM, Ge Y. 2019. Top-down Mass Spectrometry of Sarcomeric Protein Post-translational Modifications from Non-human Primate Skeletal Muscle. J. Am. Soc. Mass Spectrom.
Lin Z, Wei L, Cai W, Zhu Y, Tucholski T, et al. 2019. Simultaneous Quantification of Protein Expression and Modifications by Top-down Targeted Proteomics: A Case of the Sarcomeric Subproteome. Molecular & Cellular Proteomics. 18(3):594–605
Wang Z, Liu X, Muther J, James JA, Smith K, Wu S. 2019. Top-down Mass Spectrometry Analysis of Human Serum Autoantibody Antigen-Binding Fragments. Scientific Reports. 9(1):2345
Liang Y, Jin Y, Wu Z, Tucholski T, Brown KA, et al. 2019. Bridged Hybrid Monolithic Column Coupled to High-Resolution Mass Spectrometry for Top-Down Proteomics. Anal. Chem. 91(3):1743–47
Lącki MK, Lermyte F, Miasojedow B, Startek MP, Sobott F, et al. 2019. masstodon: A tool for assigning peaks and modeling electron transfer reactions in top-down mass spectrometry. Anal. Chem.
Lesne J, Bousquet M-P, Marcoux J, Locard-Paulet M. 2019. Top-Down and Intact Protein Mass Spectrometry Data Visualization for Proteoform Analysis Using VisioProt-MS. Bioinform Biol Insights. 13:1177932219868223
Lakshmanan R, Loo JA. 2019. Top-down protein identification using a time-of-flight mass spectrometer and data independent acquisition. International Journal of Mass Spectrometry. 435:136–44
Toby TK, Fornelli L, Srzentić K, DeHart CJ, Levitsky J, et al. 2019. A comprehensive pipeline for translational top-down proteomics from a single blood draw. Nature Protocols. 14(1):119
Sekera ER, Wood TD. 2019. Sequencing Proteins from Bottom to Top: Combining Techniques for Full Sequence Analysis of Glucokinase. In Advancements of Mass Spectrometry in Biomedical Research, eds. AG Woods, CC Darie, pp. 111–19. Cham: Springer International Publishing
Schaffer LV, Millikin RJ, Miller RM, Anderson LC, Fellers RT, et al. 2019. Identification and Quantification of Proteoforms by Mass Spectrometry. PROTEOMICS. 19(10):1800361
Schaffer LV, Millikin RJ, Miller RM, Anderson LC, Fellers RT, et al. 2019. Back Cover: Identification and Quantification of Proteoforms by Mass Spectrometry. PROTEOMICS. 19(10):1970085
Vialaret J, Lehmann S, Hirtz C. 2019. Intact Protein Analysis by LC-MS for Characterizing Biomarkers in Cerebrospinal Fluid. In Proteomics for Biomarker Discovery: Methods and Protocols, eds. V Brun, Y Couté, pp. 163–72. New York, NY: Springer New York
Coelho Graça D, Hartmer R, Jabs W, Scherl A, Clerici L, et al. 2019. Detection of Proteoforms Using Top-Down Mass Spectrometry and Diagnostic Ions. In Proteomics for Biomarker Discovery: Methods and Protocols, eds. V Brun, Y Couté, pp. 173–83. New York, NY: Springer New York
Boomathi Pandeswari P, Sabareesh V. 2019. Middle-down approach: a choice to sequence and characterize proteins/proteomes by mass spectrometry. RSC Advances. 9(1):313–44


Huilin L. 2018. Native top-down proteomics comes into light. Sci. China Life Sci.
Srzentic K, Nagornov KO, Fornelli L, Lobas AA, Ayoub D, et al. 2018. Multiplexed Middle-Down Mass Spectrometry Reveals Light and Heavy Chain Connectivity in a Monoclonal Antibody. Anal. Chem.
Yang R, Zhu D. 2018. A graph-based filtering method for top-down mass spectral identification. BMC Genomics. 19(7):666
Manconi B, Liori B, Cabras T, Vincenzoni F, Iavarone F, et al. 2018. Top-down proteomic profiling of human saliva in multiple sclerosis patients. Journal of Proteomics. 187:212–22
Wongkongkathep P, Han JY, Choi TS, Yin S, Kim HI, Loo JA. 2018. Native Top-Down Mass Spectrometry and Ion Mobility MS for Characterizing the Cobalt and Manganese Metal Binding of α-Synuclein Protein. J. Am. Soc. Mass Spectrom. 29(9):1870–80
Ghezellou P, Garikapati V, Kazemi SM, Strupat K, Ghassempour A, Spengler B. 2018. A perspective view of top-down proteomics in snake venom research. Rapid Communications in Mass Spectrometry. 0(ja):
Padiglia A, Orrù R, Boroumand M, Olianas A, Manconi B, et al. 2018. Extensive Characterization of the Human Salivary Basic Proline-Rich Proteins Family by Top-Down Mass Spectrometry. J. Proteome Res.
Cai W, Hite ZL, Lyu B, Wu Z, Lin Z, et al. 2018. Temperature-sensitive sarcomeric protein post-translational modifications revealed by top-down proteomics. Journal of Molecular and Cellular Cardiology
Riley NM, Sikora JW, Seckler HS, Greer JB, Fellers RT, et al. 2018. The Value of Activated Ion Electron Transfer Dissociation for High-Throughput Top-Down Characterization of Intact Proteins. Anal. Chem.
Krupke A, Chen C-H, Feng H, Guo R, Li P, et al. 2018. Intact NIST Monoclonal Antibody Characterization - Proteoforms, Glycoforms - using CE-MS and CE-LIF. Cogent Chemistry. 1480455
Floris F, Chiron L, Lynch A, Barrow MP, Delsuc M-A, O'Connor PB. 2018. Top-down deep sequencing of Ubiquitin using two-dimensional mass spectrometry. Anal. Chem.
Gargano AFG, Roca LS, Fellers RT, Bocxe M, Domínguez Vega E, Somsen GW. 2018. Capillary HILIC-MS: a new tool for sensitive top-down proteomics. Anal. Chem.
dos Santos Seckler H, Fornelli L, Mutharasan RK, Thaxton CS, Fellers RT, et al. 2018. A Targeted, Differential Top-Down Proteomic Methodology for Comparison of ApoA-I Proteoforms in Individuals with High and Low HDL Efflux Capacity. J. Proteome Res.
Jiang T, Hoover ME, Holt MV, Freitas MA, Marshall AG, Young NL. 2018. Middle-Down Characterization of the Cell Cycle Dependence of Histone H4 Post-Translational Modifications and Proteoforms. Proteomics. 0(0):1700442
Kachuk C, Doucette AA. 2018. The benefits (and misfortunes) of SDS in top-down proteomics. Journal of Proteomics. 175:75–86
Schmit P-O, Vialaret J, Wessels HJCT, van Gool AJ, Lehmann S, et al. 2018. Towards a routine application of Top-Down approaches for label-free discovery workflows. Journal of Proteomics. 175:12–26
Smith LM, Kelleher NL. 2018. Proteoforms as the next proteomics currency. Science. 359(6380):1106–7
Chen D, Gomes F, Abeykoon D, Lemma B, Wang Y, et al. 2018. Top-Down Analysis of Branched Proteins Using Mass Spectrometry. Anal. Chem.
LeDuc RD, Schwämmle V, Shortreed MR, Cesnik AJ, Solntsev SK, et al. 2018. ProForma: A Standard Proteoform Notation. J. Proteome Res. 17(3):1321–25
Aebersold R, Agar JN, Amster IJ, Baker MS, Bertozzi CR, et al. 2018. How many human proteoforms are there? Nature Chemical Biology. 14(3):206–14
Davis RG, Park H-M, Kim K, Greer JB, Fellers RT, et al. 2018. Top-Down Proteomics Enables Comparative Analysis of Brain Proteoforms Between Mouse Strains. Anal. Chem.
Delcourt V, Franck J, Quanico J, Gimeno J-P, Wisztorski M, et al. 2018. Spatially-Resolved Top-down Proteomics Bridged to MALDI MS Imaging Reveals the Molecular Physiome of Brain Regions. Mol Cell Proteomics. 17(2):357–72
Breuker K. 2018. Mass spectrometry: Raw protein from the top down. Nature Chemistry. 10(2):114
Polasky DA, Lermyte F, Nshanian M, Sobott F, Andrews PC, et al. 2018. Fixed-charge Trimethyl Pyrilium Modification Enables Enhanced Top-down Mass Spectrometry Sequencing of Intact Protein Complexes. Anal. Chem.
Garabedian A, Baird MA, Porter J, Jeanne Dit Fouque K, Shliaha PV, et al. 2018. Linear and Differential Ion Mobility Separations of Middle-Down Proteoforms. Anal. Chem.
Cesnik AJ, Shortreed MR, Schaffer LV, Knoener RA, Frey BL, et al. 2018. Proteoform Suite: Software for Constructing, Quantifying, and Visualizing Proteoform Families. J. Proteome Res. 17(1):568–78
Regnier FE, Kim J. 2018. Proteins and Proteoforms: New Separation Challenges. Anal. Chem. 90(1):361–73
Gomes F, Lemma B, Abeykoon D, Chen D, Wang Y, et al. 2018. Top-down Analysis of Novel Synthetic Branched Proteins. Journal of Mass Spectrometry. 0(ja):
Locard-Paulet M, Parra J, Albigot R, Mouton-Barbosa E, Bardi L, et al. 2018. VisioProt-MS: interactive 2D maps from intact protein mass spectrometry. Bioinformatics
Wu H, Xiao K, Tian Z. 2018. Top-down characterization of chicken core histones. Journal of Proteomics
Vialaret J, Schmit P-O, Lehmann S, Gabelle A, Wood J, et al. 2018. Identification of multiple proteoforms biomarkers on clinical samples by routine Top-Down approaches. Data in Brief
Why Should We Weigh Every Protein in the Human Body? 2018. News Medical.


Schaffer LV, Shortreed MR, Cesnik AJ, Frey BL, Solntsev SK, et al. 2017. Expanding Proteoform Identifications in Top-Down Proteomic Analyses by Constructing Proteoform Families. Anal. Chem.
Chang H-Y, Chen C-T, Ko C-L, Chen Y-J, Chen Y-J, et al. 2017. iTop-Q: an intelligent tool for top-down proteomics quantitation us-ing DYAMOND algorithm. Anal. Chem.
Chen B, Brown KA, Lin Z, Ge Y. 2017. Top-down Proteomics: Ready for Prime Time? Anal. Chem.
Skinner OS, Haverland NA, Fornelli L, Melani RD, Vale LHFD, et al. 2017. Top-down characterization of endogenous protein complexes with native proteomics. Nature Chemical Biology. nchembio.2515
Manconi B, Liori B, Cabras T, Vincenzoni F, Iavarone F, et al. 2017. Salivary Cystatins: Exploring New Post-Translational Modifications and Polymorphisms by Top-Down High-Resolution Mass Spectrometry. J. Proteome Res. 16(11):4196–4207
Cai W, Zhang J, Lange WJ de, Gregorich Z, Ralphe JC, et al. 2017. Top-down proteomics for assessing the maturation of stem cell-derived cardiomyocytes. Journal of Molecular and Cellular Cardiology. 112:165–66
Melani RD, Nogueira FCS, Domont GB. 2017. It is time for top-down venomics. Journal of Venomous Animals and Toxins including Tropical Diseases. 23:44
Lyon YA, Riggs D, Fornelli L, Compton PD, Julian RR. 2017. The Ups and Downs of Repeated Cleavage and Internal Fragment Production in Top-Down Proteomics. J. Am. Soc. Mass Spectrom. 1–8
Chen B, Guo X, Tucholski T, Lin Z, McIlwain S, Ge Y. 2017. The Impact of Phosphorylation on Electron Capture Dissociation of Proteins: A Top-Down Perspective. J. Am. Soc. Mass Spectrom. 28(9):1805–14
Kar UK, Simonian M, Whitelegge JP. 2017. Integral membrane proteins: bottom-up, top-down and structural proteomics. Expert Review of Proteomics. 14(8):715–23
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