Here are a number of relevant publications on top-down methods and applications.  Use the search box or browse all below.

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2023

Bhattacharya S, Joshi S, Rathore AS. 2023. A native multi-dimensional monitoring workflow for at-line characterization of mAb titer, size, charge, and glycoform heterogeneities in cell culture supernatant. Journal of Chromatography A. 463983
Ercan H, Resch U, Hsu F, Mitulovic G, Bileck A, et al. 2023. A Practical and Analytical Comparative Study of Gel-Based Top-Down and Gel-Free Bottom-Up Proteomics Including Unbiased Proteoform Detection. Cells. 12(5):747
Blöchl C, Gstöttner C, Sénard T, Stork EM, Scherer HU, et al. 2023. A robust nanoscale RP HPLC-MS approach for sensitive Fc proteoform profiling of IgG allotypes. Analytica Chimica Acta. 341795
Gabant G, Stekovic M, Nemcic M, Pinêtre J, Cadene M. 2023. A sDOE (Simple Design-of-Experiment) Approach for Parameter Optimization in Mass Spectrometry. Part 1. Parameter Selection and Interference Effects in Top-Down ETD Fragmentation of Proteins in a UHR-QTOF Instrument. J. Am. Soc. Mass Spectrom. 34(1):27–35
Contini C, Fadda L, Lai G, Masala C, Olianas A, et al. 2023. A top-down proteomic approach reveals a salivary protein profile able to classify Parkinson's disease with respect to Alzheimer's disease patients and to healthy controls. PROTEOMICS. 2300202
Schwenzer A, Kruse L, Jooß K, Neusüß C. 2023. Capillary electrophoresis‐mass spectrometry for protein analyses under native conditions: Current progress and perspectives. Proteomics. 2300135
Wang Q, Xu T, Fang F, Wang Q, Lundquist P, Sun L. 2023. Capillary Zone Electrophoresis-Tandem Mass Spectrometry for Top-Down Proteomics of Mouse Brain Integral Membrane Proteins. Anal. Chem.
Bashyal A, Dunham SD, Brodbelt JS. 2023. Characterization of Unbranched Ubiquitin Tetramers by Combining Ultraviolet Photodissociation with Proton Transfer Charge Reduction Reactions. Anal. Chem.
Habeck T, Maciel EVS, Kretschmer K, Lermyte F. 2023. Charge site manipulation to enhance top-down fragmentation efficiency. Proteomics. n/a(n/a):2300082
Tabb DL, Jeong K, Druart K, Gant MS, Brown KA, et al. 2023. Comparing Top-Down Proteoform Identification: Deconvolution, PrSM Overlap, and PTM Detection. J. Proteome Res. 22(7):2199–2217
Lanzillotti M, Brodbelt JS. 2023. Comparison of Top-Down Protein Fragmentation Induced by 213 and 193 nm UVPD. J. Am. Soc. Mass Spectrom.
Xu T, Wang Q, Wang Q, Sun L. 2023. Coupling High-Field Asymmetric Waveform Ion Mobility Spectrometry with Capillary Zone Electrophoresis-Tandem Mass Spectrometry for Top-Down Proteomics. Anal. Chem.
Chapman EA, Aballo TJ, Melby JA, Zhou T, Price SJ, et al. 2023. Defining the Sarcomeric Proteoform Landscape in Ischemic Cardiomyopathy by Top-Down Proteomics. J. Proteome Res.
Lantz C, Schrader R, Meeuwsen J, Shaw J, Goldberg NT, et al. 2023. Digital Quadrupole Isolation and Electron Capture Dissociation on an Extended Mass Range Q-TOF Provides Sequence and Structure Information on Proteins and Protein Complexes. J. Am. Soc. Mass Spectrom.
Sun Y, Liang Y, Wang C, Zhao B, Liang Z, et al. 2023. Enhancing Top-Down Characterization of Membrane Proteoforms with C8-Functional Amine-Bridged Hybrid Monoliths. Anal. Chem.
Berthias F, Cooper-Shepherd DA, Holck FHV, Langridge JI, Jensen ON. 2023. Full Separation and Sequencing of Isomeric Proteoforms in the Middle-Down Mass Range Using Cyclic Ion Mobility and Electron Capture Dissociation. Anal. Chem.
Melby JA, Brown KA, Gregorich ZR, Roberts DS, Chapman EA, et al. 2023. High sensitivity top–down proteomics captures single muscle cell heterogeneity in large proteoforms. Proceedings of the National Academy of Sciences. 120(19):e2222081120
Taylor BC, Young NL. 2023. Histone H4 proteoforms and post-translational modifications in the Mus musculus brain with quantitative comparison of ages and brain regions. Anal Bioanal Chem
McGee JP, Melani RD, Des Soye B, Croote D, Winton V, et al. 2023. Immunocomplexed Antigen Capture and Identification by Native Top-Down Mass Spectrometry. J. Am. Soc. Mass Spectrom.
Zhang Y, Cai Q, Luo Y, Zhang Y, Li H. 2023. Integrated top-down and bottom-up proteomics mass spectrometry for the characterization of endogenous ribosomal protein heterogeneity. Journal of Pharmaceutical Analysis. 13(1):63–72
Oluwole A, Shutin D, Bolla JR. 2023. Mass spectrometry of intact membrane proteins: shifting towards a more native-like context. Essays in Biochemistry. EBC20220169
Guo Y, Cupp-Sutton KA, Zhao Z, Anjum S, Wu S. 2023. Multidimensional separations in top–down proteomics. Analytical Science Advances. n/a(n/a):
Brown KA, Gugger MK, Yu Z, Moreno D, Jin S, Ge Y. 2023. Nonionic, Cleavable Surfactant for Top-Down Proteomics. Anal. Chem.
Guo Y, Chowdhury T, Seshadri M, Cupp-Sutton KA, Wang Q, et al. 2023. Optimization of Higher-Energy Collisional Dissociation Fragmentation Energy for Intact Protein-Level Tandem Mass Tag Labeling. J. Proteome Res.
Lloyd-Jones C, dos Santos Seckler H, DiStefano N, Sniderman A, Compton PD, et al. 2023. Preparative Electrophoresis for HDL Particle Size Separation and Intact-Mass Apolipoprotein Proteoform Analysis. J. Proteome Res.
You J, Park H-M. 2023. Progress in Top-Down LC-MS Analysis of Antibodies: Review. Biotechnol Bioproc E. 28(1):226–33
Kellie JF, Schneck NA, Causon JC, Baba T, Mehl JT, Pohl KI. 2023. Top-Down Characterization and Intact Mass Quantitation of a Monoclonal Antibody Drug from Serum by Use of a Quadrupole TOF MS System Equipped with Electron-Activated Dissociation. J. Am. Soc. Mass Spectrom. 34(1):17–26
Liu FC, Ridgeway ME, Wootton CA, Theisen A, Panczyk EM, et al. 2023. Top-Down Protein Analysis by Tandem-Trapped Ion Mobility Spectrometry/Mass Spectrometry (Tandem-TIMS/MS) Coupled with Ultraviolet Photodissociation (UVPD) and Parallel Accumulation/Serial Fragmentation (PASEF) MS/MS Analysis. J. Am. Soc. Mass Spectrom.
Olianas A, Guadalupi G, Cabras T, Contini C, Serrao S, et al. 2023. Top-Down Proteomics Detection of Potential Salivary Biomarkers for Autoimmune Liver Diseases Classification. International Journal of Molecular Sciences. 24(2):959
Martin EA, Fulcher JM, Zhou M, Monroe ME, Petyuk VA. 2023. TopPICR: A Companion R Package for Top-Down Proteomics Data Analysis. J. Proteome Res.

2022

Dai Y, Millikin RJ, Rolfs Z, Shortreed MR, Smith LM. 2022. A Hybrid Spectral Library and Protein Sequence Database Search Strategy for Bottom-Up and Top-Down Proteomic Data Analysis. J. Proteome Res.
Luo RY, Wong C, Xia JQ, Glader B, Shi R-Z, Zehnder JL. 2022. Accurate Identification of Hemoglobin Variants By Top-Down Protein Analysis Using Capillary Electrophoresis-High-Resolution Mass Spectrometry. Blood. 140(Supplement 1):5384–86
DiStefano N, Lloyd-Jones C, Seckler H, Compton P, Sniderman A, et al. 2022. Adaptation of Native GELFrEE for HDL Particle Size Subtype Separation and Differential Apolipoprotein Proteoform Quantification. The FASEB Journal. 36(S1):
Xu T, Han L, Sun L. 2022. Automated Capillary Isoelectric Focusing-Mass Spectrometry with Ultrahigh Resolution for Characterizing Microheterogeneity and Isoelectric Points of Intact Protein Complexes. Anal. Chem.
Fornelli L, Toby TK. 2022. Characterization of large intact protein ions by mass spectrometry: What directions should we follow? Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics. 1870(4):140758
Lin Y, Agarwal AM, Marshall AG, Anderson LC. 2022. Characterization of Structural Hemoglobin Variants by Top-Down Mass Spectrometry and R Programming Tools for Rapid Identification. J. Am. Soc. Mass Spectrom. 33(1):123–30
Cramer DAT, Franc V, Caval T, Heck AJR. 2022. Charting the Proteoform Landscape of Serum Proteins in Individual Donors by High-Resolution Native Mass Spectrometry. Anal. Chem.
Brodbelt JS. 2022. Deciphering combinatorial post-translational modifications by top-down mass spectrometry. Current Opinion in Chemical Biology. 70:102180
Babović M, Shliaha PV, Gibb S, Jensen ON. 2022. Effective Amino Acid Sequencing of Intact Filgrastim by Multimodal Mass Spectrometry with Topdownr. J. Am. Soc. Mass Spectrom.
Shaw JB, Cooper-Shepherd DA, Hewitt D, Wildgoose JL, Beckman JS, et al. 2022. Enhanced Top-Down Protein Characterization with Electron Capture Dissociation and Cyclic Ion Mobility Spectrometry. Anal. Chem.
Chen W, McCool EN, Sun L, Zang Y, Ning X, Liu X. 2022. Evaluation of Machine Learning Models for Proteoform Retention and Migration Time Prediction in Top-Down Mass Spectrometry. J. Proteome Res.
Jeong K, Babović M, Gorshkov V, Kim J, Jensen ON, Kohlbacher O. 2022. FLASHIda enables intelligent data acquisition for top–down proteomics to boost proteoform identification counts. Nat Commun. 13(1):4407
Rojas Ramírez C, Murtada R, Gao J, Ruotolo BT. 2022. Free Radical-Based Sequencing for Native Top-Down Mass Spectrometry. J. Am. Soc. Mass Spectrom.
Su P, McGee JP, Durbin KR, Hollas MAR, Yang M, et al. 2022. Highly multiplexed, label-free proteoform imaging of tissues by individual ion mass spectrometry. Science Advances. 8(32):eabp9929
Almuslehi MSM, Sen MK, Shortland PJ, Mahns DA, Coorssen JR. 2022. Histological and Top-Down Proteomic Analyses of the Visual Pathway in the Cuprizone Demyelination Model. J Mol Neurosci
Dunham SD, Wei B, Lantz C, Loo JA, Brodbelt JS. 2022. Impact of Internal Fragments on Top-Down Analysis of Intact Proteins by 193 nm UVPD. J. Proteome Res.
Kaulich PT, Cassidy L, Winkels K, Tholey A. 2022. Improved Identification of Proteoforms in Top-Down Proteomics Using FAIMS with Internal CV Stepping. Anal. Chem.
Watts E, Thyer R, Ellington AD, Brodbelt JS. 2022. Integrated Top-Down and Bottom-Up Mass Spectrometry for Characterization of Diselenide Bridging Patterns of Synthetic Selenoproteins. Anal. Chem.
Zhang Y, Cai Q, Luo Y, Zhang Y, Li H. 2022. Integrated top-down and bottom-up proteomics mass spectrometry for the characterization of endogenous ribosomal protein heterogeneity. Journal of Pharmaceutical Analysis
Macias LA, Brodbelt JS. 2022. Investigation of Product Ions Generated by 193 nm Ultraviolet Photodissociation of Peptides and Proteins Containing Disulfide Bonds. J. Am. Soc. Mass Spectrom.
Wang Q, Sun L, Lundquist PK. 2022. Large-scale top-down proteomics of the Arabidopsis thaliana leaf and chloroplast proteomes. PROTEOMICS. n/a(n/a):2100377
Adams LM, DeHart CJ, Drown BS, Anderson LC, Bocik W, et al. 2022. Mapping the KRAS Proteoform Landscape in Colorectal Cancer Identifies Truncated KRAS4B that Decreases MAPK Signaling. Journal of Biological Chemistry. 0(0):
Blevins MS, Juetten KJ, James VK, Butalewicz JP, Escobar EE, et al. 2022. Nanohydrophobic Interaction Chromatography Coupled to Ultraviolet Photodissociation Mass Spectrometry for the Analysis of Intact Proteins in Low Charge States. J. Proteome Res.
Hale OJ, Hughes JW, Sisley EK, Cooper HJ. 2022. Native Ambient Mass Spectrometry Enables Analysis of Intact Endogenous Protein Assemblies up to 145 kDa Directly from Tissue. Anal. Chem. 94(14):5608–14
Hale OJ, Cooper HJ. 2022. Native Ambient Mass Spectrometry of an Intact Membrane Protein Assembly and Soluble Protein Assemblies Directly from Lens Tissue. Angewandte Chemie International Edition. 61(31):e202201458
Harvey SR, O'Neale C, Schey KL, Wysocki VH. 2022. Native Mass Spectrometry and Surface Induced Dissociation Provide Insight into the Post-Translational Modifications of Tetrameric AQP0 Isolated from Bovine Eye Lens. Anal. Chem.
Jooß K, McGee JP, Kelleher NL. 2022. Native Mass Spectrometry at the Convergence of Structural Biology and Compositional Proteomics. Acc. Chem. Res.
Liu R, Xia S, Li H. 2022. Native top-down mass spectrometry for higher-order structural characterization of proteins and complexes. Mass Spectrometry Reviews. n/a(n/a):e21793
Wu D, Robinson CV. 2022. Native Top-Down Mass Spectrometry Reveals a Role for Interfacial Glycans on Therapeutic Cytokine and Hormone Assemblies. Angewandte Chemie International Edition. n/a(n/a):
Lantz C, Wei B, Zhao B, Jung W, Goring AK, et al. 2022. Native Top-Down Mass Spectrometry with Collisionally Activated Dissociation Yields Higher-Order Structure Information for Protein Complexes. J. Am. Chem. Soc.
Burnum-Johnson KE, Conrads TP, Drake RR, Herr AE, Iyengar R, et al. 2022. New Views of Old Proteins: Clarifying the Enigmatic Proteome. Molecular & Cellular Proteomics. 21(7):
Johnson KR, Gao Y, Greguš M, Ivanov AR. 2022. On-capillary Cell Lysis Enables Top-down Proteomic Analysis of Single Mammalian Cells by CE-MS/MS. Anal. Chem.
Rossetti DV, Inserra I, Nesticò A, Vincenzoni F, Iavarone F, et al. 2022. Pediatric Brain Tumors: Signatures from the Intact Proteome. International Journal of Molecular Sciences. 23(6):3196
Zhan Z, Wang L. 2022. Proteoform identification based on top-down tandem mass spectra with peak error corrections. Briefings in Bioinformatics. bbab599
Yang M, Hu H, Su P, Thomas PM, Camarillo JM, et al. 2022. Proteoform-Selective Imaging of Tissues Using Mass Spectrometry. Angewandte Chemie International Edition. n/a(n/a):
Habeck T, Lermyte F. 2022. Seeing the complete picture: proteins in top-down mass spectrometry. Essays in Biochemistry. EBC20220098
Takemori A, Kaulich PT, Cassidy L, Takemori N, Tholey A. 2022. Size-Based Proteome Fractionation through Polyacrylamide Gel Electrophoresis Combined with LC–FAIMS–MS for In-Depth Top-Down Proteomics. Anal. Chem.
Fornelli L, Ayoub D, Srzentic K, Nagornov K, Kozhinov A, et al. 2022. Structural Analysis of Monoclonal Antibodies with Top-down and Middledown Electron Transfer Dissociation Mass Spectrometry: The First Decade. CHIMIA. 76(1–2):114–114
Liu FC, Kirk SR, Caldwell KA, Pedrete T, Meier F, Bleiholder C. 2022. Tandem Trapped Ion Mobility Spectrometry/Mass Spectrometry (tTIMS/MS) Reveals Sequence-Specific Determinants of Top-Down Protein Fragment Ion Cross Sections. Anal. Chem.
Jeanne Dit Fouque K, Miller SA, Pham K, Bhanu NV, Cintron-Diaz YL, et al. 2022. Top-"Double-Down" Mass Spectrometry of Histone H4 Proteoforms: Tandem Ultraviolet-Photon and Mobility/Mass-Selected Electron Capture Dissociations. Anal. Chem.
Kellie JF, Schneck NA, Causon JC, Baba T, Mehl JT, Pohl KI. 2022. Top-Down Characterization and Intact Mass Quantitation of a Monoclonal Antibody Drug from Serum by Use of a Quadrupole TOF MS System Equipped with Electron-Activated Dissociation. J. Am. Soc. Mass Spectrom.
Meier-Credo J, Preiss L, Wüllenweber I, Resemann A, Nordmann C, et al. 2022. Top-Down Identification and Sequence Analysis of Small Membrane Proteins Using MALDI-MS/MS. J. Am. Soc. Mass Spectrom.
McCool EN, Lubeckyj RA, Chen D, Sun L. 2022. Top-Down Proteomics by Capillary Zone Electrophoresis-Tandem Mass Spectrometry for Large-Scale Characterization of Proteoforms in Complex Samples. In Capillary Electrophoresis-Mass Spectrometry : Methods and Protocols, eds. C Neusüß, K Jooß, pp. 107–24. New York, NY: Springer US
Polák M, Yassaghi G, Kavan D, Filandr F, Fiala J, et al. 2022. Utilization of Fast Photochemical Oxidation of Proteins and Both Bottom-up and Top-down Mass Spectrometry for Structural Characterization of a Transcription Factor–dsDNA Complex. Anal. Chem.
Rangel DL, Melani RD, Carvalho EL, Boldo JT, Gomes dos Santos T, et al. 2022. Venom characterization of the Brazilian Pampa snake Bothrops pubescens by top-down and bottom-up proteomics. Toxicon. 220:106937

2021

Xu T, Sun L. 2021. A Mini Review on Capillary Isoelectric Focusing-Mass Spectrometry for Top-Down Proteomics. Front Chem. 9:
Weisbrod CR, Anderson LC, Hendrickson CL, Schaffer LV, Shortreed MR, et al. 2021. Advanced Strategies for Proton-Transfer Reactions Coupled with Parallel Ion Parking on a 21 T FT-ICR MS for Intact Protein Analysis. Anal. Chem.
Rommelfanger SR, Zhou M, Shaghasi H, Tzeng S-C, Evans BS, et al. 2021. An Improved Top-Down Mass Spectrometry Characterization of Chlamydomonas reinhardtii Histones and Their Post-translational Modifications. J. Am. Soc. Mass Spectrom.
Shen X, Xu T, Hakkila B, Hare M, Wang Q, et al. 2021. Capillary Zone Electrophoresis-Electron-Capture Collision-Induced Dissociation on a Quadrupole Time-of-Flight Mass Spectrometer for Top-Down Characterization of Intact Proteins. J. Am. Soc. Mass Spectrom.
Chen D, Yang Z, Shen X, Sun L. 2021. Capillary Zone Electrophoresis-Tandem Mass Spectrometry As an Alternative to Liquid Chromatography-Tandem Mass Spectrometry for Top-down Proteomics of Histones. Anal. Chem.
Lantz C, Zenaidee MA, Wei B, Hemminger Z, Ogorzalek Loo RR, Loo JA. 2021. ClipsMS: An Algorithm for Analyzing Internal Fragments Resulting from Top-Down Mass Spectrometry. J. Proteome Res. 20(4):1928–35
Schaffer LV, Anderson LC, Butcher DS, Shortreed MR, Miller RM, et al. 2021. Construction of Human Proteoform Families from 21 Tesla Fourier Transform Ion Cyclotron Resonance Mass Spectrometry Top-Down Proteomic Data. J. Proteome Res. 20(1):317–25
Gerbasi VR, Melani RD, Abbatiello SE, Belford MW, Huguet R, et al. 2021. Deeper Protein Identification Using Field Asymmetric Ion Mobility Spectrometry in Top-Down Proteomics. Anal. Chem.
Borges Lima D, Dupré M, Mariano Santos MD, Carvalho PC, Chamot-Rooke J. 2021. DiagnoTop: A Computational Pipeline for Discriminating Bacterial Pathogens without Database Search. J. Am. Soc. Mass Spectrom.
Nagornov KO, Gasilova N, Kozhinov AN, Virta P, Holm P, et al. 2021. Drug-to-Antibody Ratio Estimation via Proteoform Peak Integration in the Analysis of Antibody–Oligonucleotide Conjugates with Orbitrap Fourier Transform Mass Spectrometry. Anal. Chem.
Barbu IM, Lamers R-JAN, Gerritsen HW, Blokland MH, Bremer MGEG, Alewijn M. 2021. Endogenous protein and peptide analysis with LC-MS/(MS): A feasibility study for authentication of raw-milk farmer's cheese. International Dairy Journal. 117:104990
Holt MV, Wang T, Young NL. 2021. Expeditious Extraction of Histones from Limited Cells or Tissue Samples and Quantitative Top-Down Proteomic Analysis. Current Protocols. 1(2):e26
Melby JA, de Lange WJ, Zhang J, Roberts DS, Mitchell SD, et al. 2021. Functionally Integrated Top-Down Proteomics for Standardized Assessment of Human Induced Pluripotent Stem Cell-Derived Engineered Cardiac Tissues. J. Proteome Res.
Beckman JS, Voinov VG, Hare M, Sturgeon D, Vasil'ev Y, et al. 2021. Improved Protein and PTM Characterization with a Practical Electron-Based Fragmentation on Q-TOF Instruments. J. Am. Soc. Mass Spectrom.
Schmitt ND, Berger JM, Conway JB, Agar JN. 2021. Increasing Top-Down Mass Spectrometry Sequence Coverage by an Order of Magnitude through Optimized Internal Fragment Generation and Assignment. Anal. Chem.
Boroumand M, Manconi B, Serrao S, Iavarone F, Olianas A, et al. 2021. Investigation by top-down high-performance liquid chromatography–mass spectrometry of glutathionylation and cysteinylation of salivary S100A9 and cystatin B in preterm newborns. SEPARATION SCIENCE PLUS. n/a(n/a):
Lubeckyj RA, Sun L. 2021. Laser capture microdissection-capillary zone electrophoresis-tandem mass spectrometry (LCM-CZE-MS/MS) for spatially resolved top-down proteomics: a pilot study of zebrafish brain. Mol. Omics
Lu L, Scalf M, Shortreed MR, Smith LM. 2021. Mesh Fragmentation Improves Dissociation Efficiency in Top-down Proteomics. J. Am. Soc. Mass Spectrom.
Kaulich PT, Winkels K, Kaulich TB, Treitz C, Cassidy L, Tholey A. 2021. MSTopDiff: A Tool for the Visualization of Mass Shifts in Deconvoluted Top-Down Proteomics Data for the Database-Independent Detection of Protein Modifications. J. Proteome Res.
Melby JA, Roberts DS, Larson EJ, Brown KA, Bayne EF, et al. 2021. Novel Strategies to Address the Challenges in Top-Down Proteomics. J. Am. Soc. Mass Spectrom. 32(6):1278–94
Römer J, Stolz A, Kiessig S, Moritz B, Neusüß C. 2021. Online Top-down Mass Spectrometric Identification of CE(SDS)-separated Antibody Fragments by Two-dimensional Capillary Electrophoresis. Journal of Pharmaceutical and Biomedical Analysis. 114089
Luise A, De Cecco E, Ponzini E, Sollazzo M, Mauri P, et al. 2021. Profiling Dopamine-Induced Oxidized Proteoforms of β-synuclein by Top-Down Mass Spectrometry. Antioxidants. 10(6):893
Arauz-Garofalo G, Jodar M, Vilanova M, de la Iglesia Rodriguez A, Castillo J, et al. 2021. Protamine Characterization by Top-Down Proteomics: Boosting Proteoform Identification with DBSCAN. Proteomes. 9(2):21
Jeanne Dit Fouque K, Kaplan D, Voinov VG, Holck FHV, Jensen ON, Fernandez-Lima F. 2021. Proteoform Differentiation using Tandem Trapped Ion Mobility, Electron Capture Dissociation, and ToF Mass Spectrometry. Anal. Chem.
Brunner AM, Lössl P, Geurink PP, Ovaa H, Albanese P, et al. 2021. Quantifying Positional Isomers (QPI) by top-down mass spectrometry. Molecular & Cellular Proteomics. 100070
Winkels K, Koudelka T, Tholey A. 2021. Quantitative Top-Down Proteomics by Isobaric Labeling with Thiol-Directed Tandem Mass Tags. J. Proteome Res.
Yu D, Wang Z, Cupp-Sutton KA, Guo Y, Kou Q, et al. 2021. Quantitative Top-Down Proteomics in Complex Samples Using Protein-Level Tandem Mass Tag Labeling. J. Am. Soc. Mass Spectrom.
Schachner LF, Tran DP, Lee A, McGee JP, Jooss K, et al. 2021. Reassembling protein complexes after controlled disassembly by top-down mass spectrometry in native mode. International Journal of Mass Spectrometry. 116591
Nickerson JL, Baghalabadi V, Rajendran SRCK, Jakubec PJ, Said H, et al. 2021. Recent advances in top-down proteome sample processing ahead of MS analysis. Mass Spectrometry Reviews. n/a(n/a):
Kline JT, Mullen C, Durbin KR, Oates RN, Huguet R, et al. 2021. Sequential Ion–Ion Reactions for Enhanced Gas-Phase Sequencing of Large Intact Proteins in a Tribrid Orbitrap Mass Spectrometer. J. Am. Soc. Mass Spectrom. 32(9):2334–45
Zhang Z, Hug C, Tao Y, Bitsch F, Yang Y. 2021. Solving Complex Biologics Truncation Problems by Top-Down Mass Spectrometry. J. Am. Soc. Mass Spectrom.
Roberts DS, Mann MW, Melby JA, Larson EJ, Zhu Y, et al. 2021. Structural O-Glycoform Heterogeneity of the SARS-CoV-2 Spike Protein Receptor-Binding Domain Revealed by Native Top-Down Mass Spectrometry. bioRxiv. 2021.02.28.433291
Smith LM, Agar JN, Chamot-Rooke J, Danis PO, Ge Y, et al. 2021. The Human Proteoform Project: Defining the human proteome. Science Advances. 7(46):
Simanjuntak Y, Schamoni-Kast K, Grün A, Uetrecht C, Scaturro P. 2021. Top-Down and Bottom-Up Proteomics Methods to Study RNA Virus Biology. Viruses. 13(4):668
Lefebvre D, Fenaille F, Merda D, Blanco-Valle K, Feraudet-Tarisse C, et al. 2021. Top-Down Mass Spectrometry for Trace Level Quantification of Staphylococcal Enterotoxin A Variants. J. Proteome Res.
Lermyte F. 2021. Top-Down Mass Spectrometry of Intact Proteins and Complexes: From Rare to Routine. Chromatography Online. www.chromatographyonline.com
Nagy C, Andrási M, Hamidli N, Gyémánt G, Gáspár A. 2021. Top-down proteomic analysis of monoclonal antibodies by capillary zone electrophoresis - mass spectrometry. Journal of Chromatography Open. 100024
Fagerquist CK, Dodd CE. 2021. Top-down proteomic identification of plasmid and host proteins produced by pathogenic Escherichia coli using MALDI-TOF-TOF tandem mass spectrometry. PLOS ONE. 16(11):e0260650
Choi IK, Jiang T, Kankara SR, Wu S, Liu X. 2021. TopMSV: A Web-Based Tool for Top-Down Mass Spectrometry Data Visualization. J. Am. Soc. Mass Spectrom.
Wei B, Zenaidee MA, Lantz C, Ogorzalek Loo RR, Loo JA. 2021. Towards understanding the formation of internal fragments generated by collisionally activated dissociation for top-down mass spectrometry. Analytica Chimica Acta. 339400

2020

DeHart CJ, Anderson LC, Adams LM, Fornelli L, Hendrickson CL, Kelleher NL. 2020. Abstract A26: Precise characterization and comparison of KRAS proteoforms by top-down mass spectrometry. Mol Cancer Res. 18(5 Supplement):A26–A26
Xu T, Shen X, Yang Z, Chen D, Lubeckyj RA, et al. 2020. Automated Capillary Isoelectric Focusing-Tandem Mass Spectrometry for Qualitative and Quantitative Top-Down Proteomics. Anal. Chem.
Coradin M, Mendoza MR, Sidoli S, Alpert AJ, Lu C, Garcia BA. 2020. Bullet points to evaluate the performance of the middle-down proteomics workflow for histone modification analysis. Methods
Stolz A, Hedeland Y, Salzer L, Römer J, Heiene R, et al. 2020. Capillary Zone Electrophoresis-Top-Down Tandem Mass Spectrometry for In-Depth Characterization of Hemoglobin Proteoforms in Clinical and Veterinary Samples. Anal. Chem. 92(15):10531–39
Patrie SM, Cline EN. 2020. Chapter 17 - Top-down mass spectrometry for protein molecular diagnostics, structure analysis, and biomarker discovery. In Proteomic and Metabolomic Approaches to Biomarker Discovery (Second Edition), eds. HJ Issaq, TD Veenstra, pp. 313–26. Boston: Academic Press
Giorgetti J, Beck A, Leize-Wagner E, François Y-N. 2020. Combination of intact, middle-up and bottom-up levels to characterize 7 therapeutic monoclonal antibodies by capillary electrophoresis – mass spectrometry. Journal of Pharmaceutical and Biomedical Analysis. 113107
Gault J, Liko I, Landreh M, Shutin D, Bolla JR, et al. 2020. Combining native and 'omics' mass spectrometry to identify endogenous ligands bound to membrane proteins. Nat Methods. 17(5):505–8
Yin V, Holzscherer D, Konermann L. 2020. Delineating Heme-Mediated versus Direct Protein Oxidation in Peroxidase-Activated Cytochrome c by Top-Down Mass Spectrometry. Biochemistry
Vimer S, Ben-Nissan G, Sharon M. 2020. Direct characterization of overproduced proteins by native mass spectrometry. Nat Protoc. 1–30
Tucholski T, Cai W, Gregorich ZR, Bayne EF, Mitchell SD, et al. 2020. Distinct hypertrophic cardiomyopathy genotypes result in convergent sarcomeric proteoform profiles revealed by top-down proteomics. PNAS
Gadkari VV, Ramírez CR, Vallejo DD, Kurulugama RT, Fjeldsted JC, Ruotolo BT. 2020. Enhanced Collision Induced Unfolding and Electron Capture Dissociation of Native-like Protein Ions. Anal. Chem.
Greisch J-F, van der Laarse SAM, Heck AJR. 2020. Enhancing Top-Down Analysis Using Chromophore-Assisted Infrared Multiphoton Dissociation from (Phospho)peptides to Protein Assemblies. Anal. Chem.
McIlwain SJ, Wu Z, Wetzel M, Belongia D, Jin Y, et al. 2020. Enhancing Top-Down Proteomics Data Analysis by Combining Deconvolution Results through a Machine Learning Strategy. J. Am. Soc. Mass Spectrom.
Basharat AR, Ning X, Liu X. 2020. EnvCNN: A Convolutional Neural Network Model for Evaluating Isotopic Envelopes in Top-Down Mass-spectral Deconvolution. Anal. Chem.
Gomes FP, Diedrich JK, Saviola AJ, Memili E, Moura AA, Yates JR. 2020. EThcD and 213 nm UVPD for Top-Down Analysis of Bovine Seminal Plasma Proteoforms on Electrophoretic and Chromatographic Time Frames. Anal. Chem.
Hempel B-F, Damm M, Mrinalini, Göçmen B, Karış M, et al. 2020. Extended Snake Venomics by Top-Down In-Source Decay: Investigating the Newly Discovered Anatolian Meadow Viper Subspecies, Vipera anatolica senliki. J. Proteome Res.
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