Jana Havlikova presents Top-Down Liquid Extraction Surface Analysis Mass Spectrometry of Microbes
June 17, 2021 11:00 AM in Eastern Time (US and Canada)
We have previously shown that LESA MS is a suitable technique for top-down analysis of proteins from bacterial colonies. The main focus was always placed on direct LESA analysis without any sample preparation. Application of LESA MS was further extended to direct analysis of infected in vitro 3D skin models and ex vivo human skin samples. Recently, we coupled LESA MS to the cylindrical FAIMS device, attached to the Orbitrap Eclipse, which allowed significant improvement in number of detected and identified proteins
Jana Havlikova is at the University of Birmingham in the UK.