Prof. Si Wu presents Advances in High-Throughput Quantitative Top-Down Proteomics

May 20, 2021 11:00 AM in Eastern Time (US and Canada)

We have established a high-throughput quantitative Top-Down proteomics platform that combines high-resolution reversed-phase liquid chromatography separation (low pH or 2D high pH/low pH) and isobaric chemical tag labeling (e.g. TMT) quantitation for the analysis of complex mixtures of intact proteins. This platform has been applied to the functional analysis of intact proteoforms via the development of a Top-Down thermal proteome profiling and thermal shift assay method.

We have also developed a novel analytical tool “Spray-Capillary” for quantitative, ultra-low volume sampling (e.g., single cell samples). We have applied these techniques to the analysis of intact antibody Fab’s in systemic lupus erythematosus (SLE) patient serum samples.

Prof. Si Wu is an Associate Professor in the Department of Chemistry and Biochemistry at the University of Oklahoma