Prof. Luca Fornelli presents > When Brute Force is Not Enough: Advanced Data Acquisition Strategies for Top-Down Proteomics

Thursday, Nov 18, 2021 11:30 AM in Eastern Time (US and Canada)

For years, mass spectrometry analysis of intact proteins has suffered from important technical limitations that, on the other hand, were only marginally impacting peptide-centric proteomic approaches. Recently, this performance gap has been partially filled by the introduction of high-resolution mass analyzers, a variety of new ion activation techniques, and novel signal processing algorithms. Nonetheless, some of the old problems affecting top-down proteomics cannot be solved simply by applying these improved technological resources to standard data acquisition schemes. Therefore, to circumvent some of these issues – specifically those linked to the characterization of large proteoforms – we will describe potential solutions using alternative strategies based on ion manipulation, gas-phase fractionation and targeted data acquisition.