Mass Spectrometry Tutorials

Top Down Proteomics Lecture (Albert Heck)

Lecture from MaxQuant Summer School 2014, by Albert Heck from Utrecht University on Top Down Proteomics.

TriVersa NanoMate®: LC/MS Fraction Collection

In liquid chromatography/mass spectrometry (LC/MS) fraction collection mode, the TriVersa NanoMate allows users to couple any conventional HPLC system to the TriVersa NanoMate for MS analysis. The effluent is split post column with a portion directed to a conventional LC/MS interface while the remainder of the split effluent is directed to collect at time segments into a multi-well plate. The chromatogram is thereby "cataloged" for future analysis. Following LC/MS, the researcher is able to select fractions of interest for more in-depth analysis via MS/MS. The process gives researchers the ability to spend more time analyzing complex portions of their samples without having to reinject. LC/MS with simultaneous fraction collection is ideally suited for researchers interested in metabolite identification, biomarker discovery, and protein characterization or any application that requires more time for analysis than what is allowed during conventional LC/MS

Mag Lab Tour: Ion Cyclotron Resonance Program

ICR slices and dices molecules to reveal exactly what's in them. The last in a 10-chapter online tour of the National High Magnetic FIeld Laboratory.

FTICR-MS Tutorial

FTICR-MS: Fourier transform ion cyclotron resonance mass spectrometry. An MS configuration that detects signals on metal plates from electrical currents produced by ion oscillations.

Analysis of Intact Proteins in Complex Mixtures using Online Fragmentation

Shannon Eliuk presents this scientific poster about a complete top down proteomics workflow from sample preparation to data analysis. This robust and sensitive method for identification and characterization of proteins in a mixture uses a variety of fragmentation techniques including CID, HCD, and ETD, with a Thermo Scientific Orbitrap Elite high-field Orbitrap mass spectrometer.