Antibody Fragmentation

Fragmentation of the hinge region of IgG, producing a F(ab’)2 fragment and two Fc fragments

  • FabRICATOR® is an enzyme for preparation of F(ab')2.

    FabRICATOR® is a digestive enzyme that cleaves IgG only at one specific site below the hinge region resulting in F(ab')2 and Fc-fragments. Since FabRICATOR® only cleaves at one specific site below the hinge region, there is no risk of over digestion if the incubation time is increased for several hours.

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Fragmentation of human IgG1 between K223 and T224 producing a homogenous pool of Fab and Fc fragments

  • GingisKHAN™ (Gingipain K) is an enzyme that digests human IgG1 yielding intact Fab and Fc fragments.

    http://www.fcoss.nl/?writing-an-essay-for-college-application-week Writing An Essay For College Application Week GingisKHAN™ digests human IgG1 at one single site in the upper hinge (...KSCDK / THTCPPCP...). A second cleavage site on the Fc may appear if the N-glycans are removed. GingisKHAN™ is a cysteine protease and requires reducing environment to be active. Intact Fab and Fc fragments are obtained with GingisKHAN™ digestion of human IgG1 since mild reducing conditions (i.e. 2 mM cysteine) is sufficient for enzyme activity. Optimal activity is obtained at 37°C and pH 8. Reducing agent is supplied with the enzyme.

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