Publications

Mapping intact protein isoforms in discovery mode using top-down proteomics.

Tran JC, Zamdborg L, Ahlf DR, Lee JE, Catherman AD, Durbin KR, Tipton JD, Vellaichamy A, Kellie JF, Li M, Wu C, Sweet SM, Early BP, Siuti N, LeDuc RD, Compton PD, Thomas PM, Kelleher NL "Mapping intact protein isoforms in discovery mode using top-down proteomics." Nature, 2011, 480(7376) p. 254-8

PubMed: 22037311
PubMed Central: PMC3237778
DOI:10.1038/nature10575

Analysis of intact protein isoforms by mass spectrometry.

Tipton JD, Tran JC, Catherman AD, Ahlf DR, Durbin KR, Kelleher NL "Analysis of intact protein isoforms by mass spectrometry." J Biol Chem, 2011, 286(29) p. 25451-8

PubMed: 21632550
PubMed Central: PMC3138281
DOI:10.1074/jbc.R111.239442

Structural analysis of intact monoclonal antibodies by electron transfer dissociation mass spectrometry.

Tsybin YO, Fornelli L, Stoermer C, Luebeck M, Parra J, Nallet S, Wurm FM, Hartmer R "Structural analysis of intact monoclonal antibodies by electron transfer dissociation mass spectrometry." Anal Chem, 2011, 83(23) p. 8919-27

PubMed: 22017162
DOI:10.1021/ac201293m

Top-down mass spectrometry of supercharged native protein-ligand complexes.

Yin S, Loo JA "Top-Down Mass Spectrometry of Supercharged Native Protein-Ligand Complexes." Int J Mass Spectrom, 2011, 300(2-3) p. 118-122

PubMed: 21499519
PubMed Central: PMC3076692
DOI:10.1016/j.ijms.2010.06.032

Characterization of deamidation of barstar using electrospray ionization quadrupole time-of-flight mass spectrometry, which stabilizes an equilibrium unfolding intermediate.

Jha SK, Deepalakshmi PD, Udgaonkar JB "Characterization of deamidation of barstar using electrospray ionization quadrupole time-of-flight mass spectrometry, which stabilizes an equilibrium unfolding intermediate." Protein Sci, 2012, 21(5) p. 633-46

PubMed: 22431291
PubMed Central: PMC3403461
DOI:10.1002/pro.2047

UV Radiation Induces Genome-Mediated, Site-Specific Cleavage in Viral Proteins.

Wigginton KR, Menin L, Sigstam T, Gannon G, Cascella M, Hamidane HB, Tsybin YO, Waridel P, Kohn T "UV radiation induces genome-mediated, site-specific cleavage in viral proteins." Chembiochem, 2012, 13(6) p. 837-45

PubMed: 22416020
DOI:10.1002/cbic.201100601

Purification and High-Resolution Top-Down Mass Spectrometric Characterization of Human Salivary α-Amylase.

Peng Y, Chen X, Sato T, Rankin SA, Tsuji RF, Ge Y "Purification and high-resolution top-down mass spectrometric characterization of human salivary α-amylase." Anal Chem, 2012, 84(7) p. 3339-46

PubMed: 22390166
DOI:10.1021/ac300083y

Filter diagonalization method-based mass spectrometry for molecular and macromolecular structure analysis.

Kozhinov AN, Tsybin YO "Filter diagonalization method-based mass spectrometry for molecular and macromolecular structure analysis." Anal Chem, 2012, 84(6) p. 2850-6

PubMed: 22376180
DOI:10.1021/ac203391z

Top-Down FTICR MS for the Identification of Fluorescent Labeling Efficiency and Specificity of the Cu-Protein Azurin.

Nicolardi S, Andreoni A, Tabares LC, van der Burgt YE, Canters GW, Deelder AM, Hensbergen PJ "Top-down FTICR MS for the identification of fluorescent labeling efficiency and specificity of the Cu-protein azurin." Anal Chem, 2012, 84(5) p. 2512-20

PubMed: 22320330
DOI:10.1021/ac203370f

Top-Down Proteomics and Direct Surface Sampling of Neonatal Dried Blood Spots: Diagnosis of Unknown Hemoglobin Variants

Edwards RL, Griffiths P, Bunch J, Cooper HJ "Top-down proteomics and direct surface sampling of neonatal dried blood spots: diagnosis of unknown hemoglobin variants." J Am Soc Mass Spectrom, 2012, 23(11) p. 1921-30

PubMed: 22993042
DOI:10.1007/s13361-012-0477-9

Native Mass Spectrometry Characterization of Intact Nanodisc Lipoprotein Complexes

Marty MT, Zhang H, Cui W, Blankenship RE, Gross ML, Sligar SG "Native mass spectrometry characterization of intact nanodisc lipoprotein complexes." Anal Chem, 2012, 84(21) p. 8957-60

PubMed: 23061736
PubMed Central: PMC3536499
DOI:10.1021/ac302663f

Top-down Targeted Proteomics for Deep Sequencing of Tropomyosin Isoforms

Peng Y, Chen X, Zhang H, Xu Q, Hacker TA, Ge Y "Top-down targeted proteomics for deep sequencing of tropomyosin isoforms." J Proteome Res, 2013, 12(1) p. 187-98

PubMed: 23256820
PubMed Central: PMC3596867
DOI:10.1021/pr301054n

Application of the ETD/PTR reactions in top-down proteomics as a faster alternative to bottom-up nanoLC-MS/MS protein identification

Drabik A, Bodzon-Kulakowska A, Suder P "Application of the ETD/PTR reactions in top-down proteomics as a faster alternative to bottom-up nanoLC-MS/MS protein identification." J Mass Spectrom, 2012, 47(10) p. 1347-52

PubMed: 23019167
DOI:10.1002/jms.3086

High-resolution differential ion mobility spectrometry of a protein.

Shvartsburg AA, Smith RD "High-resolution differential ion mobility spectrometry of a protein." Anal Chem, 2013, 85(1) p. 10-3

PubMed: 23244633
PubMed Central: PMC3552553
DOI:10.1021/ac3029129

Top down proteomics of human membrane proteins from enriched mitochondrial fractions.

Catherman AD, Li M, Tran JC, Durbin KR, Compton PD, Early BP, Thomas PM, Kelleher NL "Top down proteomics of human membrane proteins from enriched mitochondrial fractions." Anal Chem, 2013, 85(3) p. 1880-8

PubMed: 23305238
PubMed Central: PMC3565750
DOI:10.1021/ac3031527

Top-Down Structural Analysis of Post-Translationally Modified Proteins by FTICR-MS with H/D Exchange and Electron Capture Dissociation.

Pan J, Borchers CH "Top-down structural analysis of posttranslationally modified proteins by Fourier transform ion cyclotron resonance-MS with hydrogen/deuterium exchange and electron capture dissociation." Proteomics, 2013, 13(6) p. 974-81

PubMed: 23319428
DOI:10.1002/pmic.201200246

Combining chemical labeling, bottom-up and top-down ion-mobility mass spectrometry to identify metal-binding sites of partially-metallated metallothionein.

Chen SH, Russell WK, Russell DH "Combining chemical labeling, bottom-up and top-down ion-mobility mass spectrometry to identify metal-binding sites of partially metalated metallothionein." Anal Chem, 2013, 85(6) p. 3229-37

PubMed: 23421923
DOI:10.1021/ac303522h

Proteoform: a single term describing protein complexity

Smith LM, Kelleher NL, Consortium for Top Down Proteomics "Proteoform: a single term describing protein complexity." Nat Methods, 2013, 10(3) p. 186-7

PubMed: 23443629
PubMed Central: PMC4114032
DOI:10.1038/nmeth.2369

Top-down Targeted Proteomics for Deep Sequencing of Tropomyosin Isoforms

Peng Y, Chen X, Zhang H, Xu Q, Hacker TA, Ge Y "Top-down targeted proteomics for deep sequencing of tropomyosin isoforms." J Proteome Res, 2013, 12(1) p. 187-98

PubMed: 23256820
PubMed Central: PMC3596867
DOI:10.1021/pr301054n

Characterization and Quantification of Intact 26S Proteasome Proteins by Real-Time Measurement of Intrinsic Fluorescence Prior to Top-down Mass Spectrometry

Russell JD, Scalf M, Book AJ, Ladror DT, Vierstra RD, Smith LM, Coon JJ "Characterization and quantification of intact 26S proteasome proteins by real-time measurement of intrinsic fluorescence prior to top-down mass spectrometry." PLoS One, 2013, 8(3) p. e58157

PubMed: 23536786
PubMed Central: PMC3594244
DOI:10.1371/journal.pone.0058157

Identification of New Apolipoprotein-CIII Glycoforms with Ultrahigh Resolution MALDI-FTICR Mass Spectrometry of Human Sera

Nicolardi S, van der Burgt YE, Dragan I, Hensbergen PJ, Deelder AM "Identification of new apolipoprotein-CIII glycoforms with ultrahigh resolution MALDI-FTICR mass spectrometry of human sera." J Proteome Res, 2013, 12(5) p. 2260-8

PubMed: 23527852
DOI:10.1021/pr400136p

Top-Down Structural Analysis of an Intact Monoclonal Antibody by Electron Capture Dissociation-Fourier Transform Ion Cyclotron Resonance-Mass Spectrometry

Mao Y, Valeja SG, Rouse JC, Hendrickson CL, Marshall AG "Top-down structural analysis of an intact monoclonal antibody by electron capture dissociation-Fourier transform ion cyclotron resonance-mass spectrometry." Anal Chem, 2013, 85(9) p. 4239-46

PubMed: 23551206
DOI:10.1021/ac303525n

Multipurpose Dissociation Cell for Enhanced ETD of Intact Protein Species

Rose CM, Russell JD, Ledvina AR, McAlister GC, Westphall MS, Griep-Raming J, Schwartz JC, Coon JJ, Syka JE "Multipurpose dissociation cell for enhanced ETD of intact protein species." J Am Soc Mass Spectrom, 2013, 24(6) p. 816-27

PubMed: 23609185
PubMed Central: PMC3667591
DOI:10.1007/s13361-013-0622-0

Top-down proteomics reveals a unique protein S-thiolation switch in Salmonella Typhimurium in response to infection-like conditions

Ansong C, Wu S, Meng D, Liu X, Brewer HM, Deatherage Kaiser BL, Nakayasu ES, Cort JR, Pevzner P, Smith RD, Heffron F, Adkins JN, Pasa-Tolic L "Top-down proteomics reveals a unique protein S-thiolation switch in Salmonella Typhimurium in response to infection-like conditions." Proc Natl Acad Sci U S A, 2013, 110(25) p. 10153-8

PubMed: 23720318
PubMed Central: PMC3690903
DOI:10.1073/pnas.1221210110

Significant Modifications of the Salivary Proteome Potentially Associated with Complications of Down Syndrome Revealed by Top-down Proteomics

Cabras T, Pisano E, Montaldo C, Giuca MR, Iavarone F, Zampino G, Castagnola M, Messana I "Significant modifications of the salivary proteome potentially associated with complications of Down syndrome revealed by top-down proteomics." Mol Cell Proteomics, 2013, 12(7) p. 1844-52

PubMed: 23533003
PubMed Central: PMC3708170
DOI:10.1074/mcp.M112.026708