Buying Papers High-throughput analysis of intact human proteins using UVPD and HCD on an Orbitrap mass spectrometer.
Dissertation Analysis Plan J Proteome Res. 2017 Apr 17;:
Write Me An Essay Cheap Online Authors: Cleland TP, DeHart CJ, Fellers RT, VanNispen AJ, Greer JB, LeDuc RD, Parker WR, Thomas PM, Kelleher NL, Brodbelt JSManipulated In Fight Club
Philosophy Essay Writers Abstract The analysis of intact proteins (top-down strategy) by mass spectrometry has great potential to elucidate proteoform variation, including patterns of post-translational modifications (PTMs), which may not be discernable by analysis of peptides alone (bottom-up approach). To maximize sequence coverage and localization of PTMs, various fragmentation modes have been developed to produce fragment ions from deep within intact proteins. Ultraviolet photodissociation (UVPD) has recently been shown to produce high sequence coverage and PTM retention on a variety of proteins, with increasing evidence of efficacy on a chromatographic time scale. However, utilization of UVPD for high-throughput top-down analysis to date has been limited by bioinformatics. Here, we detected 153 proteins and 489 proteoforms using UVPD and 271 proteins and 982 proteoforms using higher-energy collisional dissociation (HCD) in a comparative analysis of HeLa whole-cell lysate by qualitative top-down proteomics. Of the total detected proteoforms, 286 overlapped between the UVPD and HCD datasets, with 68% of proteoforms having C-scores greater than 40 for UVPD and 63% for HCD. The average sequence coverage (28% ± 20% for UVPD versus 17% ± 8% for HCD, p < 0.0001) found to be higher for UVPD than HCD, and with a trend toward improvement in q-value for the UVPD dataset. This study demonstrates the complementarity of UVPD and HCD for more extensive protein profiling and proteoform characterization. Thesis Of Phd In English
PMID: 28412815 [PubMed - as supplied by publisher]Scarlet Letter Essay